Alternative Name(s):

D-mannonate hydro-lyase

Species: (Organism)

Sorangium cellulosum (strain So ce56) (Polyangium cellulosum (strain So ce56))

Gene Names:

uxuA

Tag info:

N-terminal 6xHis-SUMO-tagged

Target Protein AA Sequence:

MKMTFRWYGENDPVKLEYIRQIPGIYGIVSAIYDVPVGEVWPVERLQALRARVEGVGLRFEVVESVPVHEDIKLGKPARERLIDNFCQNIRNCAAAGVKVICYNFMPVFDWTRTEMARELPDGSLTLAFDAAAVAEIDPEQGISLPGWDSSYRPEQLRAVLDEYKNVSEEALWEHLEHFLRRIIPVAEEVGVRMAMHPDDPPRPIFGLPRIVKNRDDLMRLVKTVDSPANGITLCSGSLGADLCNNIVSLVREFGGMGRIPFGHLRNVAVQEDGTFFESAHLSCEGSLDMAAIVKAYHDVGFDGYVRPDHGRMIWGETGKPGYGLYDRALGLVYLNGLWEATCKLSPGPAAKVPAPG

Expression Region:

1-357aa

Subcellular Location:

Tissue Specificity:

Protein Length:

Full Length

Pathway:

Mol. Weight:

55.7 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

Catalyzes the dehydration of D-mannonate.

Involvement in disease:

Relevance:

Catalyzes the dehydration of D-mannonate.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Mannonate dehydratase family

Reference:

"Complete genome sequence of the myxobacterium Sorangium cellulosum."Schneiker S., Perlova O., Kaiser O., Gerth K., Alici A., Altmeyer M.O., Bartels D., Bekel T., Beyer S., Bode E., Bode H.B., Bolten C.J., Choudhuri J.V., Doss S., Elnakady Y.A., Frank B., Gaigalat L., Goesmann A. Mueller R.Nat. Biotechnol. 25:1281-1289(2007)