Alternative Name(s):

Exo-alpha-galactosidase B1 (EC:3.2.1.22)

Species: (Organism)

Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / JCM 5826 / NBRC 14291 / NCTC 11154)

Gene Names:

glaB1

Tag info:

N-terminal 6xHis-tagged

Target Protein AA Sequence:

CTTLSAQKVYEISAFGLKANSSKNASPVLQKALAKIKAEYKEGEKVILRFPEGRYEFHEKGAAVREYYISNHDQTNPKKVGIALEDMKNLTLDGQGSEFVFHGRMLPVSLLRSENCLLKNFSIDFENPHIAQVKIVENDPQDGIVFEPAPWVDYRIAKDSIFEAYGEGWTMRHSWGIAFDGDTKHLVYNTSDIGCPTKGASEVAPRRIHAPGWKDARLVPGTVVAMRGWGRPTPGIFLSHDVNTTIENVKVHYAEGMGLLAQLCENITLEKFGVCLKGDADPRYFTTQADATHFSGCKGKIVSCNGLYEGMMDDAINVHGTYLKVVKRVDDRTLVGRYMHGQSWGFEWGCPGDEVQFIRSNTMELVGKQNKIISIRPYDKEQTEGAREFLITFQEPVDQVINEQSGFGIENLTWTPEVLFSGNVIRNNRARGSLFSTPRKTIVENNLFDHTSGAAILLCGDCNGWFETGACRHVIIRKNRFVNALTNLFQFTNAVISIYPEIPDLKGQQQYFHGGPEGGIVIEDNEFETFDAPILYAKSVDGLVFRNNTIKLNTEYKPFHPNRNRFWLERVTNVTIAE

Expression Region:

15-592aa

Subcellular Location:

Cell membrane, Lipid-anchor

Tissue Specificity:

Protein Length:

Full Length of Mature Protein

Pathway:

Mol. Weight:

69 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

Alpha-galactosidase. Removes both branched alpha-1,3-linked galactose residues of blood group B antigens and linear alpha-1,3-linked galactose structures.

Involvement in disease:

Relevance:

Alpha-galactosidase. Roves both branched alpha-1,3-linked galactose residues of blood group B antigens and linear alpha-1,3-linked galactose structures .

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Glycosyl hydrolase 110 family, B subfamily

Reference:

Evolution of symbiotic bacteria in the distal human intestine.Xu J., Mahowald M.A., Ley R.E., Lozupone C.A., Hamady M., Martens E.C., Henrissat B., Coutinho P.M., Minx P., Latreille P., Cordum H., Van Brunt A., Kim K., Fulton R.S., Fulton L.A., Clifton S.W., Wilson R.K., Knight R.D., Gordon J.I.PLoS Biol. 5:1574-1586(2007)