Alternative Name(s):

Formamide amidohydrolase

Species: (Organism)

Bradyrhizobium sp. (strain ORS278)

Gene Names:

amiF

Tag info:

N-terminal 6xHis-SUMO-tagged

Target Protein AA Sequence:

MNGLGGLNKSEHGVVIGLVQLQLPVVVTKEDLAKQTEKIVWMVGKARRNLGTMDLVVFPEYSLHGLSMDTNPEIMCRLDGPEVAAFKQACIDNKIWGCFSIMEYNPDGNPYNSGLIIDSNGEIKLYYRKLHPWIPVEPWEPGDLGIPVIEGPRGAKIALIICHDGMFPEMARECAYKGAEIMIRTAGYTAPIRDSWRFTNQANAFQNLMVTANVCMCGSDGSFDSMGEGMIVNFDGSILAHGTTGRADEIITAEVRPDLVREARIGWGVENNIYQLWHRGYVAVKGGAMDCPYTFMHDMVAGTYRLPWEDQVKITDGTSCGFPAPTRVFGKMAKAAE

Expression Region:

1-337aa

Subcellular Location:

Tissue Specificity:

Protein Length:

Full Length

Pathway:

Mol. Weight:

53.2 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

Is an aliphatic amidase with a restricted substrate specificity, as it only hydrolyzes formamide.

Involvement in disease:

Relevance:

Is an aliphatic amidase with a restricted substrate specificity, as it only hydrolyzes formamide.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Carbon-nitrogen hydrolase superfamily, Aliphatic amidase family

Reference:

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia."Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. Sadowsky M.Science 316:1307-1312(2007)