Alternative Name(s):

Arginine esterase; EC 3.4.21.35

Species: (Organism)

Canis lupus familiaris (Dog) (Canis familiaris)

Gene Names:

N/A

Tag info:

N-terminal 6xHis-SUMO-tagged

Target Protein AA Sequence:

IIGGRECLKNSQPWQVAVYHNGEFACGGVLVNPEWVLTAAHCANSNCEVWLGRHNLSESEDEGQLVQVRKSFIHPLYKTKVPRAVIRPGEDRSHDLMLLHLEEPAKITKAVRVMDLPKKEPPLGSTCYVSGWGSTDPETIFHPGSLQCVDLKLLSNNQCAKVYTQKVTKFMLCAGVLEGKKDTCKGDSGGPLICDGELVGITSWGATPCGKPQMPSLYTRVMPHLMWIKDTMKANT

Expression Region:

25-260aa

Subcellular Location:

Tissue Specificity:

Protein Length:

Full Length of Mature Protein

Pathway:

Mol. Weight:

42 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

This serine protease is found in dog seminal plasma, its exact physiological function is not known.

Involvement in disease:

Relevance:

This serine protease is found in dog seminal plasma, its exact physiological function is not known. Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Peptidase S1 family, Kallikrein subfamily

Reference:

"Nucleotide sequence of the androgen-dependent arginine esterase mRNA of canine prostate."Chapdelaine P., Ho-Kim M.-A., Tremblay R.R., Dube J.Y.FEBS Lett. 232:187-192(1988)