Alternative Name(s):

nlpD; b2742; JW2712Murein hydrolase activator NlpD

Species: (Organism)

Escherichia coli (strain K12)

Gene Names:

nlpD

Tag info:

N-terminal 6xHis-SUMO-tagged

Target Protein AA Sequence:

CSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR

Expression Region:

26-379aa

Subcellular Location:

Cell inner membrane, Lipid-anchor

Tissue Specificity:

Protein Length:

Full Length of Mature Protein

Pathway:

Mol. Weight:

53.5 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Microbiology

Function:

Activator of the cell wall hydrolase AmiC. Required for septal murein cleavage and daughter cell separation during cell division.

Involvement in disease:

Relevance:

Activator of the cell wall hydrolase AmiC. Required for septal murein cleavage and daughter cell separation during cell division.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

E.coli NlpD/Haemophilus LppB family

Reference:

"LytM-domain factors are required for daughter cell separation and rapid ampicillin-induced lysis in Escherichia coli."Uehara T., Dinh T., Bernhardt T.G.J. Bacteriol. 191:5094-5107(2009).