Alternative Name(s):

rpmC; b3312; JW3274; 50S ribosomal protein L29; Large ribosomal subunit protein uL29

Species: (Organism)

Escherichia coli (strain K12)

Gene Names:

rpmC

Tag info:

N-terminal GST-tagged

Target Protein AA Sequence:

MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLKQVRRDVARVKTLLNEKAGA

Expression Region:

1-63aa

Subcellular Location:

Tissue Specificity:

Protein Length:

Full Length

Pathway:

Mol. Weight:

34.3 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

Binds 23S rRNA. It is not essential for growth.

Involvement in disease:

Relevance:

Binds 23S rRNA. It is not essential for growth.One of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit. Contacts trigger factor .

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Universal ribosomal protein uL29 family

Reference:

Interplay of signal recognition particle and trigger factor at L23 near the nascent chain exit site on the Escherichia coli ribosome.Ullers R.S., Houben E.N.G., Raine A., ten Hagen-Jongman C.M., Ehrenberg M., Brunner J., Oudega B., Harms N., Luirink J.J. Cell Biol. 161:679-684(2003)