Alternative Name(s):

MEL (MLT) (Allergen Api m 3) (Allergen Api m III) (Allergen: Api m 4)

Species: (Organism)

Apis mellifera (Honeybee)

Gene Names:

MELT

Tag info:

Tag-Free

Target Protein AA Sequence:

GIGAVLKVLTTGLPALISWIKRKRQQ

Expression Region:

44-69aa

Subcellular Location:

Tissue Specificity:

Protein Length:

Full Length of Mature Protein

Pathway:

Mol. Weight:

2.8 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Cell Biology

Function:

Involvement in disease:

Relevance:

Melittin: Main toxin of bee venom with strong hemolytic activity and antimicrobial activity . It has enhancing effects on bee venom phospholipase A2 activity . This amphipathic toxin binds to negatively charged membrane surface and forms pore by inserting into lipid bilayers inducing the leakage of ions and molecules and the enhancement of permeability that ultimately leads to cell lysis . It acts as a voltage-gated pore with higher selectivity for anions over cations . The ion conductance has been shown to be voltage-dependent . Self-association of melittin in membranes is promoted by high ionic strength, but not by the presence of negatively charged lipids . In vivo, intradermal injection into healthy human volunteers produce sharp pain sensation and an inflammatory response . It produces pain by activating primary nociceptor cells directly and indirectly due to its ability to activate plasma membrane phospholipase A2 and its pore-forming activity .

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Reference:

"Identification of a novel melittin isoform from Africanized Apis mellifera venom." Sciani J.M., Marques-Porto R., Lourenco A. Jr., Orsi R.D., Junior R.S., Barraviera B., Pimenta D.C. Peptides 31:1473-1479(2010)