Alternative Name(s):

ponA; DDB_G0293522; Ponticulin

Species: (Organism)

Dictyostelium discoideum (Slime mold)

Gene Names:

ponA

Tag info:

N-terminal 6xHis-tagged

Target Protein AA Sequence:

QYTLSVSNSASGSKCTTAVSAKLNACNTGCLNSFNIVESSNGKGLVFKTFINAACSGEYESLSQFTCAANQKIPTTSYIVSCNSTPSSNSTTDSDS

Expression Region:

23-118aa

Subcellular Location:

Cell membrane, Multi-pass membrane protein, Cell membrane, Lipid-anchor, GPI-anchor

Tissue Specificity:

Protein Length:

Full Length of Mature Protein

Pathway:

Mol. Weight:

13.9 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Microbiology

Function:

Binds F-actin and nucleates actin assembly. Major high affinity link between the plasma membrane and the cortical actin network.

Involvement in disease:

Relevance:

Binds F-actin and nucleates actin assembly. Major high affinity link between the plasma membrane and the cortical actin network.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Ponticulin family

Reference:

"Identification of a second member of the ponticulin gene family and its differential expression pattern."Hitt A.L., Iijima-Shimizu M., DuBay M.J., Antonette L.L., Urushihara H., Wilkerson C.G.Biochim. Biophys. Acta 1628:79-87(2003).