Alternative Name(s):

tcyA; yckK; BSU03610; L-cystine-binding protein TcyA

Species: (Organism)

Bacillus subtilis (strain 168)

Gene Names:

tcyA

Tag info:

N-terminal 6xHis-SUMO-tagged

Target Protein AA Sequence:

CGAGNDNQSKDNAKDGDLWASIKKKGVLTVGTEGTYEPFTYHDKDTDKLTGYDVEVITEVAKRLGLKVDFKETQWDSMFAGLNSKRFDVVANQVGKTDREDKYDFSDKYTTSRAVVVTKKDNNDIKSEADVKGKTSAQSLTSNYNKLATNAGAKVEGVEGMAQALQMIQQGRVDMTYNDKLAVLNYLKTSGNKNVKIAFETGEPQSTYFTFRKGSGEVVDQVNKALKEMKEDGTLSKISKKWFGEDVSK

Expression Region:

20-268aa

Subcellular Location:

Cell membrane, Lipid-anchor

Tissue Specificity:

Protein Length:

Full Length of Mature Protein

Pathway:

Mol. Weight:

43.6 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

Part of the ABC transporter complex TcyABC involved in L-cystine import.

Involvement in disease:

Relevance:

Part of the ABC transporter complex TcyABC involved in L-cystine import.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Bacterial solute-binding protein 3 family

Reference:

Three different systems participate in L-cystine uptake in Bacillus subtilis.Burguiere P., Auger S., Hullo M.-F., Danchin A., Martin-Verstraete I.J. Bacteriol. 186:4875-4884(2004)