Alternative Name(s):

Transcription factor xGATA-1A

Species: (Organism)

Xenopus laevis (African clawed frog)

Gene Names:

gata1-a

Tag info:

N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged

Target Protein AA Sequence:

MDYTTLTTQDPDPNYTESGLASTSEDSQFLYGLGGESSPGHYGGAVSSRAVGGFRHSPVFQTFPLHWPETSAGIPSNLTAYGRSTGTLSFYPSAASALGPITSPPLYSASSFLLGSAPPAEREGSPKFLETLKTERASPLTSDLLPLEPRSPSILQVGYIGGGGQEFSLFQSTEDRECVNCGATVTPLWRRDMSGHYLCNACGLYHKMNGQNRPLIRPKKRLIVSKRAGTQCSNCHTSTTTLWRRNASGDPVCNACGLYYKLHNVNRPLTMKKEGIQTRNRKVSSRSKKKKQLDNPFEPPKAGVEEPSPYPFGPLLFHGQMPPMGHMINPPHHFLQSPRISHSAPAVSYRQAASGVTPP

Expression Region:

1-359aa

Subcellular Location:

Nucleus

Tissue Specificity:

Expressed in the developing ventral blood island, and in both tadpole and adult erythrocytes.

Protein Length:

Full Length

Pathway:

Mol. Weight:

59 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Epigenetics and Nuclear Signaling

Function:

Transcription factor that acts synergistically with tal1/scl and lmo2 to specify embryonic dorsal mesoderm to a hematopoietic fate.

Involvement in disease:

Relevance:

Transcription factor that acts synergistically with tal1/scl and lmo2 to specify embryonic dorsal mesoderm to a hematopoietic fate.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Reference:

"Expression of GATA-binding proteins during embryonic development in Xenopus laevis." Zon L.I., Mather C., Burgess S., Bolce M.E., Harland R.M., Orkin S.H. Proc. Natl. Acad. Sci. U.S.A. 88:10642-10646(1991)