Alternative Name(s):

Nucleocapsid protein Protein N

Species: (Organism)

Junin mammarenavirus (JUNV) (Junn mammarenavirus)

Gene Names:

N

Tag info:

N-terminal 6xHis-SUMO-tagged

Target Protein AA Sequence:

MAHSKEVPSFRWTQSLRRGLSQFTQTVKSDVLKDAKLIADSIDFNQVAQVQRALRKTKRGEEDLNKLRDLNKEVDRLMSMRSVQRNTVFKAGDLGRVERMELASGLGNLKTKFRRAETGSQGVYMGNLSQSQLAKRSEILRTLGFQQQGTGGNGVVRVWDVKDPSKLNNQFGSVPALTIACMTVQGGETMNSVIQALTSLGLLYTVKYPNLSDLDRLTQEHDCLQIVTKDESSINISGYNFSLSAAVKAGASILDDGNMLETIRVTPDNFSSLIKSTIQVKRREGMFIDEKPGNRNPYENLLYKLCLSGDGWPYIGSRSQIIGRSWDNTSIDLTRKPVAGPRQPEKNGQNLRLANLTEIQEAVIREAVGKLDPTNTLWLDIEGPATDPVEMALFQPAGSKYIHCFRKPHDEKGFKNGSRHSHGILMKDIEDAMPGVLSYVIGLLPPDMVVTTQGSDDIRKLFDLHGRRDLKLVDVRLTSEQARQFDQQVWEKFGHLCKHHNGVVVSKKKRDKDAPFKLASSEPHCALLDCIMFQSVLDGKLYEEELTPLLPPSLLFLPKAAYAL

Expression Region:

1-564aa

Subcellular Location:

Virion, Host cytoplasm

Tissue Specificity:

Protein Length:

Full Length

Pathway:

Mol. Weight:

79 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Disables the host innate defense by interfering with beta interferon (IFNB) production through the inhibition of host IRF3 phosphorylation and activation by host IKBKE. Through the interaction with host IKBKE, strongly inhibits the phosphorylation and nuclear translocation of IRF3, a protein involved in IFN activation pathway, leading to the inhibition of IFNB and IRF3-dependent promoters activation (By similarity). Interacts with host IKBKE (via Protein kinase domain); the interaction inhibits IKBKE kinase activity.

Involvement in disease:

Relevance:

Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Disables the host innate defense by interfering with beta interferon (IFNB) production through the inhibition of host IRF3 phosphorylation and activation by host IKBKE. Through the interaction with host IKBKE, strongly inhibits the phosphorylation and nuclear translocation of IRF3, a protein involved in IFN activation pathway, leading to the inhibition of IFNB and IRF3-dependent promoters activation (By similarity). Interacts with host IKBKE (via Protein kinase domain); the interaction inhibits IKBKE kinase activity.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Arenaviridae nucleocapsid protein family

Reference:

"Molecular organization of Junin virus S RNA: complete nucleotide sequence, relationship with other members of the Arenaviridae and unusual secondary structures."Ghiringhelli P.D., Rivera-Pomar R.V., Lozano M.E., Grau O., Romanowski V.J. Gen. Virol. 72:2129-2141(1991)