Alternative Name(s):

Short name:Fas-2 Short name:Fas2 Alternative name(s): Acetylcholinesterase toxin F-VII Fasciculin-II Short name:FAS-II Toxin TA1

Species: (Organism)

Dendroaspis angusticeps (Eastern green mamba) (Naja angusticeps)

Gene Names:

Fas-2

Tag info:

N-terminal 6xHis-SUMO-tagged

Target Protein AA Sequence:

TMCYSHTTTSRAILTNCGENSCYRKSRRHPPKMVLGRGCGCPPGDDNLEVKCCTSPDKCNY

Expression Region:

1-61aa

Subcellular Location:

Secreted

Tissue Specificity:

Expressed by the venom gland.

Protein Length:

Full Length

Pathway:

Mol. Weight:

22.8 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

Interferes with neuromuscular transmission by inhibiting the enzyme acetylcholinesterase (AChE) present at the neuromuscular junction. It selectively binds and inhibits with a 1

Involvement in disease:

Relevance:

Interferes with neuromuscular transmission by inhibiting the enzyme acetylcholinesterase (AChE) present at the neuromuscular junction. It selectively binds and inhibits with a 1:1 stoichiometry the mammalian and electric fish AChE at picomolar concentrations. It is highly specific for the peripheral site of AChE and blocks the entry of acetylcholine into the active site of the enzyme (through the Met-33 residue), thereby preventing its breakdown. It has been called fasciculin since after injection into mice it causes severe, generalized and long-lasting (5-7 hours) fasciculations.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Snake three-finger toxin family, Short-chain subfamily, Acn-esterase inhibitor sub-subfamily

Reference:

"Snake venom toxins. The purification and amino acid sequence of toxin F-VII from Dendroaspis angusticeps venom."Viljoen C.C., Botes D.P.J. Biol. Chem. 248:4915-4919(1973)