Alternative Name(s):

Ribosome-inactivating protein PD-L1/PD-L2; EC 3.2.2.22; rRNA N-glycosidase PD-L1/PD-L2

Species: (Organism)

Phytolacca dioica (Bella sombra tree) (Phytolacca arborea)

Gene Names:

N/A

Tag info:

N-terminal 6xHis-SUMO-tagged

Target Protein AA Sequence:

INTITYDAGNTTINKYATFMESLRNEAKDPSLQCYGIPMLPNNSSTIKYLLVKLQGASQKTITLMLRRNNLYVMGYSDPFNGNCRYHIFNDITGTERTNVENTLCSSSSSRDAKPINYNSLYSTLEKKAEVNSRSQVQLGIQILSSDIGKISGQSSFTDKTEAKFLLVAIQMVSEAARFKYIENQVKTNFNRDFSPNDKILDLEENWGKISTAIHDATNGALPKPLELKNADGTKWIVLRVDEIKPDMGLLNYVNGTCQTT

Expression Region:

1-261aa

Subcellular Location:

Tissue Specificity:

Protein Length:

Full Length

Pathway:

Mol. Weight:

45.2 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

Inhibits protein synthesis. Cleaves supercoiled pBR322 dsDNA.

Involvement in disease:

Relevance:

Inhibits protein synthesis. Cleaves supercoiled pBR322 dsDNA.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Ribosome-inactivating protein family, Type 1 RIP subfamily

Reference:

Structural characterization and comparative modeling of PD-Ls 1-3, type 1 ribosome-inactivating proteins from summer leaves of Phytolacca dioica L.Di Maro A., Chambery A., Carafa V., Costantini S., Colonna G., Parente A.Biochimie 91:352-363(2009)