Alternative Name(s):

yegS; b2086; JW2070; Lipid kinase YegS; EC 2.7.1.-

Species: (Organism)

Escherichia coli (strain K12)

Gene Names:

yegS

Tag info:

N-terminal 6xHis-SUMO-tagged

Target Protein AA Sequence:

MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKGDAARYVEEARKFGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATSVGIPEALDKALKLAIAGDAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALGSVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALINDGLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHDITFNLDGEPLSGQNFHIEILPAALRCRLPPDCPLLR

Expression Region:

1-299aa

Subcellular Location:

Cytoplasm

Tissue Specificity:

Protein Length:

Full Length

Pathway:

Mol. Weight:

48 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Microbiology

Function:

In vitro phosphorylates phosphatidylglycerol but not diacylglycerol; the in vivo substrate is unknown.

Involvement in disease:

Relevance:

In vitro phosphorylates phosphatidylglycerol but not diacylglycerol; the in vivo substrate is unknown.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Diacylglycerol/lipid kinase family, YegS lipid kinase subfamily

Reference:

"Expression, purification, crystallization and preliminary diffraction studies of the mammalian DAG kinase homologue yegS from Escherichia coli."Bakali H.M.A., Nordlund P., Hallberg B.M.Acta Crystallogr. F 62:295-297(2006)