Alternative Name(s):

fliC; flaF; hag; b1923; JW1908Flagellin

Species: (Organism)

Escherichia coli (strain K12)

Gene Names:

fliC

Tag info:

N-terminal 6xHis-SUMO-tagged

Target Protein AA Sequence:

AQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDDAAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRVRELTVQATTGTNSESDLSSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKNGSMKIQVGANDNQTITIDLKQIDAKTLGLDGFSVKNNDTVTTSAPVTAFGATTTNNIKLTGITLSTEAATDTGGTNPASIEGVYTDNGNDYYAKITGGDNDGKYYAVTVANDGTVTMATGATANATVTDANTTKATTITSGGTPVQIDNTAGSATANLGAVSLVKLQDSKGNDTDTYALKDTNGNLYAADVNETTGAVSVKTITYTDSSGAASSPTAVKLGGDDGKTEVVDIDGKTYDSADLNGGNLQTGLTAGGEALTAVANGKTTDPLKALDDAIASVDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG

Expression Region:

2-498aa

Subcellular Location:

Secreted, Bacterial flagellum

Tissue Specificity:

Protein Length:

Full Length of Mature protein

Pathway:

Mol. Weight:

67.2 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Immunology

Function:

Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.

Involvement in disease:

Relevance:

Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Bacterial flagellin family

Reference:

"Benchmarking yeast two-hybrid systems using the interactions of bacterial motility proteins." Rajagopala S.V., Hughes K.T., Uetz P. Proteomics 9:5296-5302(2009)