Alternative Name(s):

Protein melted homolog (Veph)

Species: (Organism)

Mus musculus (Mouse)

Gene Names:

Veph1

Tag info:

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Target Protein AA Sequence:

AMTESTFPQQKDLEQLQLHLEEVRFFDVFGFSETAGAWQCFMCNNPEKATVVNQDGQPLIEGKLKEKQVRWKFIKRWKTHYFTLAGNQLLFQKGKSKDDPDDSPIELSKVQSVKAVAKKRRDRSLPRAFEIFTDSKTYVFKAKDEKNAEEWLQCINVALAQAKERESREVTTYL

Expression Region:

660-833aa

Subcellular Location:

Cell membrane, Peripheral membrane protein, Cytoplasmic side

Tissue Specificity:

Specifically expressed in kidney and eye. In the eye, expressed in retinal pigmented epithelium but not in the neural retina.

Protein Length:

Partial

Pathway:

Mol. Weight:

27.7 kDa

Purity:

Greater than 85% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

Interacts with TGF-beta receptor type-1 (TGFBR1) and inhibits dissociation of activated SMAD2 from TGFBR1, impeding its nuclear accumulation and resulting in impaired TGF-beta signaling. May also affect FOXO, Hippo and Wnt signaling.

Involvement in disease:

Relevance:

Interacts with TGF-beta receptor type-1 (TGFBR1) and inhibits dissociation of activated SMAD2 from TGFBR1, impeding its nuclear accumulation and resulting in impaired TGF-beta signaling. May also affect FOXO, Hippo and Wnt signaling.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

MELT/VEPH family

Reference:

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004)