Alternative Name(s):

Ste20-like kinase;Sterile 20/oxidant stress-response kinase 1 ;SOK-1 ;Ste20/oxidant stress response kinase 1

Species: (Organism)

Homo sapiens (Human)

Gene Names:

STK25

Tag info:

N-terminal 6xHis-SUMO-tagged

Target Protein AA Sequence:

MAHLRGFANQHSRVDPEELFTKLDRIGKGSFGEVYKGIDNHTKEVVAIKIIDLEEAEDEIEDIQQEITVLSQCDSPYITRYFGSYLKSTKLWIIMEYLGGGSALDLLKPGPLEETYIATILREILKGLDYLHSERKIHRDIKAANVLLSEQGDVKLADFGVAGQLTDTQIKRNTFVGTPFWMAPEVIKQSAYDFKADIWSLGITAIELAKGEPPNSDLHPMRVLFLIPKNSPPTLEGQHSKPFKEFVEACLNKDPRFRPTAKELLKHKFITRYTKKTSFLTELIDRYKRWKSEGHGEESSSEDSDIDGEAEDGEQGPIWTFPPTIRPSPHSKLHKGTALHSSQKPAEPVKRQPRSQCLSTLVRPVFGELKEKHKQSGGSVGALEELENAFSLAEESCPGISDKLMVHLVERVQRFSHNRNHLTSTR

Expression Region:

1-426aa

Subcellular Location:

Cytoplasm, Golgi apparatus

Tissue Specificity:

Ubiquitously expressed. Highest levels are found in testis, large intestine, brain and stomach followed by heart and lung.

Protein Length:

Full Length

Pathway:

Mol. Weight:

64.1 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Signal Transduction

Function:

Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration.

Involvement in disease:

Relevance:

Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Protein kinase superfamily, STE Ser/Thr protein kinase family, STE20 subfamily

Reference:

Activation of a human Ste20-like kinase by oxidant stress defines a novel stress response pathway.Pombo C.M., Bonventre J.V., Molnar A., Kyriakis J., Force T.EMBO J. 15:4537-4546(1996)