Alternative Name(s):

Super protein ;SUPSynaptosomal-associated 25KDA protein

Species: (Organism)

Homo sapiens (Human)

Gene Names:

SNAP25

Tag info:

N-terminal 6xHis-tagged

Target Protein AA Sequence:

MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLDRVEEGMNHINQDMKEAEKNLKDLGKCCGLFICPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG

Expression Region:

1-206aa

Subcellular Location:

Cytoplasm, perinuclear region, Cell membrane, Lipid-anchor, Cell junction, synapse, synaptosome

Tissue Specificity:

Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.

Protein Length:

Full Length of Isoform 2

Pathway:

Excitatorysynapsepathway

Mol. Weight:

27.3 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Neuroscience

Function:

t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.

Involvement in disease:

Myasthenic syndrome, congenital, 18 (CMS18)

Relevance:

t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systs. Associates with proteins involved in vesicle docking and mbrane fusion. Regulates plasma mbrane recycling through its interaction with CENPF.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

SNAP-25 family

Reference:

Human cDNA clones encoding two different isoforms of the nerve terminal protein SNAP-25.Bark I.C., Wilson M.C.Gene 139:291-292(1994)