Alternative Name(s):

Excitatory amino-acid carrier 1 Neuronal and epithelial glutamate transporter Sodium-dependent glutamate/aspartate transporter 3 Solute carrier family 1 member 1

Species: (Organism)

Homo sapiens (Human)

Gene Names:

SLC1A1

Tag info:

N-terminal GST-tagged

Target Protein AA Sequence:

AEDVTLIIAVDWLLDRFRTMVNVLGDAFGTGIVEKLSKKELEQMDVSSEVNIVNPFALESTILDNEDSDTKKSYVNGGFAVDKSDTISFTQTSQF

Expression Region:

430-524aa

Subcellular Location:

Cell membrane, Multi-pass membrane protein, Apical cell membrane, Multi-pass membrane protein

Tissue Specificity:

Expressed in all tissues tested including liver, muscle, testis, ovary, retinoblastoma cell line, neurons and brain (in which there was dense expression in substantia nigra, red nucleus, hippocampus and in cerebral cortical layers).

Protein Length:

Partial

Pathway:

Glutamatergicsynapse

Mol. Weight:

37.5 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Neuroscience

Function:

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate

Involvement in disease:

Dicarboxylic aminoaciduria (DCBXA); Schizophrenia 18 (SCZD18)

Relevance:

Transports L-glutamate, L- and D-aspartate and L-cystein (PubMed:21123949). Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Negatively regulated by ARL6IP5

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family, SLC1A1 subfamily

Reference:

"Loss-of-function mutations in the glutamate transporter SLC1A1 cause human dicarboxylic aminoaciduria."Bailey C.G., Ryan R.M., Thoeng A.D., Ng C., King K., Vanslambrouck J.M., Auray-Blais C., Vandenberg R.J., Broer S., Rasko J.E.J. Clin. Invest. 121:446-453(2011)