Alternative Name(s):

Opsin-2

Species: (Organism)

Homo sapiens (Human)

Gene Names:

RHO

Tag info:

N-terminal 6xHis-SUMO-tagged

Target Protein AA Sequence:

MNGTEGPNFYVPFSNATGVVRSPFEYPQYYLAEPWQ

Expression Region:

1-36aa

Subcellular Location:

Membrane, Multi-pass membrane protein, Cell projection, cilium, photoreceptor outer segment

Tissue Specificity:

Rod shaped photoreceptor cells which mediate vision in dim light.

Protein Length:

Partial

Pathway:

Mol. Weight:

20.2 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Signal Transduction

Function:

Photoreceptor required for image-forming vision at low light intensity

Involvement in disease:

Retinitis pigmentosa 4 (RP4); Night blindness, congenital stationary, autosomal dominant 1 (CSNBAD1)

Relevance:

Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

G-protein coupled receptor 1 family, Opsin subfamily

Reference:

"Isolation and nucleotide sequence of the gene encoding human rhodopsin."Nathans J., Hogness D.S.Proc. Natl. Acad. Sci. U.S.A. 81:4851-4855(1984)