Alternative Name(s):

Ca(2+)-dependent phospholipid scramblase 1 Erythrocyte phospholipid scramblase MmTRA1b

Species: (Organism)

Homo sapiens (Human)

Gene Names:

PLSCR1

Tag info:

N-terminal GST-tagged

Target Protein AA Sequence:

ESTGSQEQKSGVW

Expression Region:

1-318aa

Subcellular Location:

Cell membrane, Single-pass type II membrane protein, Membrane, Lipid-anchor, Cytoplasmic side, Nucleus

Tissue Specificity:

Expressed in platelets, erythrocyte membranes, lymphocytes, spleen, thymus, prostate, testis, uterus, intestine, colon, heart, placenta, lung, liver, kidney and pancreas. Not detected in brain and skeletal muscle.

Protein Length:

Full Length

Pathway:

Mol. Weight:

28.4 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Microbiology

Function:

May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system.; FUNCTION

Involvement in disease:

Relevance:

May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system. May play a role in the antiviral response of interferon (IFN) by amplifying and enhancing the IFN response through increased expression of select subset of potent antiviral genes. May contribute to cytokine-regulated cell proliferation and differentiation.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Phospholipid scramblase family

Reference:

"Isolation of an erythrocyte membrane protein that mediates Ca2+-dependent transbilayer movement of phospholipid." Basse F., Stout J.G., Sims P.J., Wiedmer T. J. Biol. Chem. 271:17205-17210(1996)