Alternative Name(s):

GIIC sPLA2Group IIA phospholipase A2Non-pancreatic secretory phospholipase A2 ;NPS-PLA2;Phosphatidylcholine 2-acylhydrolase 2A

Species: (Organism)

Homo sapiens (Human)

Gene Names:

PLA2G2A

Tag info:

N-terminal 6xHis-tagged

Target Protein AA Sequence:

NLVNFHRMIKLTTGKEAALSYGFYGCHCGVGGRGSPKDATDRCCVTHDCCYKRLEKRGCGTKFLSYKFSNSGSRITCAKQDSCRSQLCECDKAAATCFARNKTTYNKKYQYYSNKHCRGSTPRC

Expression Region:

21-144aa

Subcellular Location:

Cell membrane, Peripheral membrane protein, Secreted

Tissue Specificity:

Protein Length:

Full Length of Mature Protein

Pathway:

Vascularsmoothmusclecontraction

Mol. Weight:

17.9 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Metabolism

Function:

Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides

Involvement in disease:

Relevance:

Thought to participate in the regulation of the phospholipid metabolism in biombranes including eicosanoid biosynthesis. Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Phospholipase A2 family

Reference:

Cloning and recombinant expression of phospholipase A2 present in rheumatoid arthritic synovial fluid.Seilhamer J.J., Pruzanski W., Vadas P., Plant S., Miller J.A., Kloss J., Johnson L.K.J. Biol. Chem. 264:5335-5338(1989)