Alternative Name(s):

Glucosaminyl N-deacetylase/N-sulfotransferase 1 ;NDST-1N-heparan sulfate sulfotransferase 1 ;N-HSST 1[Heparan sulfate]-glucosamine N-sulfotransferase 1 ;HSNST 1 2 domains:Heparan sulfate N-deacetylase 1 (EC:3.-.-.-)Heparan sulfate N-sulfotransferase 1 (EC:2.8.2.-)

Species: (Organism)

Homo sapiens (Human)

Gene Names:

NDST1

Tag info:

N-terminal 6xHis-SUMO-tagged

Target Protein AA Sequence:

GWKRGLEPSADAPEPDCGDPPPVAPSRLLPLKPVQAATPSRTDPLVLVFVESLYSQLGQEVVAILESSRFKYRTEIAPGKGDMPTLTDKGRGRFALIIYENILKYVNLDAWNRELLDKYCVAYGVGIIGFFKANENSLLSAQLKGFPLFLHSNLGLKDCSINPKSPLLYVTRPSEVEKGVLPGEDWTVFQSNHSTYEPVLLAKTRSSESIPHLGADAGLHAALHATVVQDLGLHDGIQRVLFGNNLNFWLHKLVFVDAVAFLTGKRLSLPLDRYILVDIDDIFVGKEGTRMKVEDVKALFDTQNELRAHIPNFTFNLGYSGKFFHTGTNAEDAGDDLLLSYVKEFWWFPHMWSHMQPHLFHNQSVLAEQMALNKKFAVEHGIPTDMGYAVAPHHSGVYPVHVQLYEAWKQVWSIRVTSTEEYPHLKPARYRRGFIHNGIMVLPRQTCGLFTHTIFYNEYPGGSSELDKIINGGELFLTVLLNPVSAPQPMAAGEKGLLHSLSAADTGFLEPGKGGEA

Expression Region:

40-556aa

Subcellular Location:

Golgi apparatus membrane, Single-pass type II membrane protein

Tissue Specificity:

Widely expressed. Expression is most abundant in heart, liver and pancreas.

Protein Length:

Partial of Isoform 2

Pathway:

Mol. Weight:

73.5 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Immunology

Function:

Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis

Involvement in disease:

Mental retardation, autosomal recessive 46 (MRT46)

Relevance:

Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands, thereby playing a role in inflammatory response.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Sulfotransferase 1 family, NDST subfamily

Reference:

NDST1 missense mutations in autosomal recessive intellectual disability.Reuter M.S., Musante L., Hu H., Diederich S., Sticht H., Ekici A.B., Uebe S., Wienker T.F., Bartsch O., Zechner U., Oppitz C., Keleman K., Jamra R.A., Najmabadi H., Schweiger S., Reis A., Kahrizi K.Am. J. Med. Genet. A 164A:2753-2763(2014)