Alternative Name(s):

Membrane-bound transferrin-like protein p97; CD228

Species: (Organism)

Oryctolagus cuniculus (Rabbit)

Gene Names:

Mfi2

Tag info:

N-terminal 6xHis-SUMO-tagged

Target Protein AA Sequence:

SVEVRWCTASEPEQQKCEDMSQAFREAGLQPALLCVQGTSADHCVQLIAAHEADAITLDGGAIYEAGKEHGLKPVVGEVYDQEVGTSYYAVAVVKRSSNVTINTLRGVKSCHTGINRTVGWNVPVGYLVDSGRLSVMGCDVLKAVSEYFGGSCVPGAGETRYSESLCRLCRGDTSGEGVCDKSPLERYYDYSGAFRCLAEGAGDVAFVKHSTVLENTDGRTLPSWGHMLMSRDFELLCRDGSRASVTEWQHCHLARVPAHAVVVRADTDAGLIFRLLNEGQRLFSHEGSSFQMFSSEAYGQKNLLFKDSTLELVPIATQTYEAWLGPEYLHAMKGLLCDPNRLPPYLRWCVLSTPEIQKCGDMAVAFSRQRLKPEIQCVSAESPQHCMEQIQAGHIDAVTLNGEDIHTAGKTYGLIPAAGELYAADDRSNSYFVVAVVKRDSAYAFTVDELRGKRSCHPGFGSPAGWDVPVGALIHWGYIRPRNCDVLTAVGQFFNASCVPVNNPKKYPSSLCALCVGDEQGRNKCTGNSQERYYGDSGAFRCLVEGAGDVAFVKHTTIFDNTNGHNPEPWAAHLRSQDYELLCPNGARAEAHQFAACNLAQIPSHAVMVRPDTNIFTVYGLLDKAQDLFGDDHNKNGFKMFDSSSYHGRDLLFKDATVRAVPVGERTTYQDWLGPDYVAALEGMQSQRCS

Expression Region:

20-710aa

Subcellular Location:

Cell membrane, Lipid-anchor, GPI-anchor

Tissue Specificity:

Protein Length:

Partial

Pathway:

Mol. Weight:

91.7 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc (By similarity).

Involvement in disease:

Relevance:

Involved in iron cellular uptake. Ses to be internalized and then recycled back to the cell mbrane. Binds a single atom of iron per subunit. Could also bind zinc .

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Transferrin family

Reference:

Expression of membrane-bound transferrin-like protein p97 on the cell surface of chondrocyte.Kawamoto T., Pan H., Yan W., Ishida H., Usui E., Oda R., Nakamasu K., Noshiro M., Kawashima-Ohya Y., Fujii M., Shintani H., Okada Y., Kato Y.Eur. J. Biochem. 256:503-509(1998)