Alternative Name(s):

C2orf33

Species: (Organism)

Homo sapiens (Human)

Gene Names:

MFF

Tag info:

N-terminal 6xHis-tagged

Target Protein AA Sequence:

MSKGTSSDTSLGRVSRAAFPSPTAAEMAEISRIQYEMEYTEGISQRMRVPEKLKVAPPNADLEQGFQEGVPNASVIMQVPERIVVAGNNEDVSFSRPADLDLIQSTPFKPLALKTPPRVLTLSERPLDFLDLERPPTTPQNEEIRAVGRLKRERSMSENAVRQNGQLVRNDSLWHRSDSAPRNKISRFQAPISAPEYTVTPSPQQARVCPPHMLPEDGANLSSARGILSLIQSSTRRAYQQILDVLDENRRPVLRGGSAAATSNPHHDNVRYGISNIDTTIEGTSDDLTVVDAASLRRQIIKLNRRLQLLEEENKERAKREM

Expression Region:

1-322aa

Subcellular Location:

Mitochondrion outer membrane, Single-pass type IV membrane protein, Peroxisome, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle

Tissue Specificity:

Highly expressed in heart, kidney, liver, brain, muscle, and stomach.

Protein Length:

Partial

Pathway:

Mol. Weight:

39.9 kDa

Purity:

Greater than 85% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

Plays a role in mitochondrial and peroxisomal fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. May be involved in regulation of synaptic vesicle membrane dynamics by recruitment of DNM1L to clathrin-containing vesicles.

Involvement in disease:

Encephalopathy due to defective mitochondrial and peroxisomal fission 2 (EMPF2)

Relevance:

Plays a role in mitochondrial and peroxisomal fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. May be involved in regulation of synaptic vesicle membrane dynamics by recruitment of DNM1L to clathrin-containing vesicles.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Tango11 family

Reference:

"The novel tail-anchored membrane protein Mff controls mitochondrial and peroxisomal fission in mammalian cells." Gandre-Babbe S., van der Bliek A.M. Mol. Biol. Cell 19:2402-2412(2008)