Alternative Name(s):

14.5KDA translational inhibitor protein ;p14.5Heat-responsive protein 12UK114 antigen homolog

Species: (Organism)

Homo sapiens (Human)

Gene Names:

HRSP12

Tag info:

N-terminal GST-tagged

Target Protein AA Sequence:

SSLIRRVISTAKAPGAIGPYSQAVLVDRTIYISGQIGMDPSSGQLVSGGVAEEAKQALKNMGEILKAAGCDFTNVVKTTVLLADINDFNTVNEIYKQYFKSNFPARAAYQVAALPKGSRIEIEAVAIQGPLTTASL

Expression Region:

2-137AA

Subcellular Location:

Cytoplasm, Nucleus, Peroxisome, Mitochondrion

Tissue Specificity:

Expressed predominantly in liver and kidney. Lower levels in lung and brain.

Protein Length:

Full Length of Mature Protein

Pathway:

Mol. Weight:

41.4 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Epigenetics and Nuclear Signaling

Function:

Catalyzes the hydrolytic deamination of enamine/imine intermediates that form during the course of normal metabolism. May facilitate the release of ammonia from these potentially toxic reactive metabolites, reducing their impact on cellular components. It may act on enamine/imine intermediates formed by several types of pyridoxal-5'-phosphate-dependent dehydratases including L-threonine dehydratase.

Involvement in disease:

Relevance:

Endoribonuclease responsible for the inhibition of the translation by cleaving mRNA. Inhibits cell-free protein synthesis. Cleaves phosphodiester bonds only in single-stranded RNA .

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

RutC family

Reference:

Isolation and characterization of a 14.5-KDA trichloroacetic-acid-soluble translational inhibitor protein from human monocytes that is upregulated upon cellular differentiation.Schmiedeknecht G., Kerkhoff C., Orso E., Stoehr J., Aslanidis C., Nagy G.M., Knuechel R., Schmitz G.Eur. J. Biochem. 242:339-351(1996)