Alternative Name(s):

gfapGlial fibrillary acidic protein; GFAP

Species: (Organism)

Danio rerio (Zebrafish) (Brachydanio rerio)

Gene Names:

gfap

Tag info:

N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged

Target Protein AA Sequence:

MESQRSFSSYRKRFGTPGGSPSVGVTSRHSTGRLSLHSSPRHLTSSPLTLSTSRLSLGGERLDFSADSLLKAQYRETRTNEKVEMMGLNDRFASYIEKVRFLEQQNKMLVAELNQLRGKEPSRLGDIYQEELRELRRQVDGLNAGKARLEIERDNLASDLGTLKQRLQDETALRQEAENNLNTFRQDVDEAALNRVQLERKIEALQDEINFLKKVHEEEMRELHEQLMAQQVHVDLDVSKPDLTAALKEIRAQFEAMANSNMQETEEWYRSKFADLTDAANRNGEALRQAKQEANDYRRQIQGLTCDLESLRGSNESLERQLREMEERFAIETAGYQDTVARLEDEIQMLKEEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEESRITVPVQNFTNLQFRDTSMDTKLTPEAHVKRSIVVRTVETRDGEIIKESTTERKDLP

Expression Region:

1-444aa

Subcellular Location:

Cytoplasm, cytoskeleton

Tissue Specificity:

Protein Length:

Full Length

Pathway:

Mol. Weight:

71.2 kDa

Purity:

Greater than 85% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Neuroscience

Function:

Involvement in disease:

Relevance:

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Intermediate filament family

Reference:

"Structural and functional characterization of the zebrafish gene for glial fibrillary acidic protein, GFAP." Nielsen A.L., Joergensen A.L. Gene 310:123-132(2003)