Alternative Name(s):

GCA; GCL; Grancalcin

Species: (Organism)

Homo sapiens (Human)

Gene Names:

GCA

Tag info:

N-terminal GST-tagged

Target Protein AA Sequence:

MAYPGYGGGFGNFSIQVPGMQMGQPVPETGPAILLDGYSGPAYSDTYSSAGDSVYTYFSAVAGQDGEVDAEELQRCLTQSGINGTYSPFSLETCRIMIAMLDRDHTGKMGFNAFKELWAALNAWKENFMTVDQDGSGTVEHHELRQAIGLMGYRLSPQTLTTIVKRYSKNGRIFFDDYVACCVKLRALTDFFRKRDHLQQGSANFIYDDFLQGTMAI

Expression Region:

1-217aa

Subcellular Location:

Cytoplasm, Cytoplasmic granule membrane, Peripheral membrane protein, Cytoplasmic side

Tissue Specificity:

Detected in neutrophils and macrophages (at protein level). Highly expressed in bone marrow.

Protein Length:

Full Length

Pathway:

Mol. Weight:

51 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

Calcium-binding protein that may play a role in the adhesion of neutrophils to fibronectin. May play a role in the formation of focal adhesions.

Involvement in disease:

Relevance:

Calcium-binding protein that may play a role in the adhesion of neutrophils to fibronectin. May play a role in the formation of focal adhesions.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Reference:

"Biochemical characterization of the penta-EF-hand protein grancalcin and identification of L-plastin as a binding partner." Lollike K., Johnsen A.H., Durussel I., Borregaard N., Cox J.A. J. Biol. Chem. 276:17762-17769(2001)