Alternative Name(s):

Heme synthase (Protoheme ferro-lyase)

Species: (Organism)

Bos taurus (Bovine)

Gene Names:

FECH

Tag info:

N-terminal 10xHis-SUMO-tagged and C-terminal Myc-taggedd

Target Protein AA Sequence:

SPKPQAQPGNRKPRTGILMLNMGGPETVEEVQDFLQRLFLDQDLMTLPVQDKLGPFIAKRRTPKIQEQYRRIGGGSPIKMWTSKQGEGMVKLLDELSPHTAPHKYYIGFRYVHPLTEEAIEEMERDGLERAVAFTQYPQYSCSTTGSSLNAIYRYYNEVGRKPTMKWSTIDRWPTHPLLIQCFADHILKELDHFPPEKRREVVILFSAHSLPMSVVNRGDPYPQEVGATVQRVMDKLGYSNPYRLVWQSKVGPMPWLGPQTDEAIKGLCKRGRKNILLVPIAFTSDHIETLYELDIEYSQVLASECGLENIRRAESLNGNPLFSKALADLVHSHLQSKERCSTQLTLSCPLCVNPTCRETKSFFTSQQL

Expression Region:

48-416aa

Subcellular Location:

Tissue Specificity:

Protein Length:

Full Length of Mature Protein

Pathway:

Mol. Weight:

58.1 kDa

Purity:

Greater than 85% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Cardiovascular

Function:

Involvement in disease:

Relevance:

Catalyzes the ferrous insertion into protoporphyrin IX.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Reference:

"Molecular cloning, sequencing, and expression of mouse ferrochelatase." Taketani S., Nakahashi Y., Osumi T., Tokunaga R. J. Biol. Chem. 265:19377-19380(1990)