Alternative Name(s):

Bone marrow serine protease;Elastase-2;Human leukocyte elastase ;HLEMedullasin;PMN elastase

Species: (Organism)

Homo sapiens (Human)

Gene Names:

ELANE

Tag info:

N-terminal GST-tagged

Target Protein AA Sequence:

IVGGRRARPHAWPFMVSLQLRGGHFCGATLIAPNFVMSAAHCVANVNVRAVRVVLGAHNLSRREPTRQVFAVQRIFENGYDPVNLLNDIVILQLNGSATINANVQVAQLPAQGRRLGNGVQCLAMGWGLLGRNRGIASVLQELNVTVVTSLCRRSNVCTLVRGRQAGVCFGDSGSPLVCNGLIHGIASFVRGGCASGLYPDAFAPVAQFVNWIDSIIQRSEDNPCPHPRDPDPASRTH

Expression Region:

30-267aa

Subcellular Location:

Tissue Specificity:

Bone marrow cells.

Protein Length:

Full Length of Mature Protein

Pathway:

Mol. Weight:

52.6 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Signal Transduction

Function:

Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.

Involvement in disease:

Cyclic haematopoiesis (CH); Neutropenia, severe congenital 1, autosomal dominant (SCN1)

Relevance:

Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chotaxis.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Peptidase S1 family, Elastase subfamily

Reference:

The spectrum of ELANE mutations and their implications in severe congenital and cyclic neutropenia.Germeshausen M., Deerberg S., Peter Y., Reimer C., Kratz C.P., Ballmaier M.Hum. Mutat. 34:905-914(2013)