Alternative Name(s):

Cryptdin-1 Defensin-related cryptdin peptide

Species: (Organism)

Mus musculus (Mouse)

Gene Names:

Defa1

Tag info:

N-terminal 6xHis-GST-tagged

Target Protein AA Sequence:

LRDLVCYCRSRGCKGRERMNGTCRKGHLLYTLCCR

Expression Region:

59-93aa

Subcellular Location:

Secreted

Tissue Specificity:

Paneth cells of the small bowel.

Protein Length:

Full Length of Mature Protein

Pathway:

Mol. Weight:

34.1 kDa

Purity:

Greater than 85% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Immunology

Function:

Probably contributes to the antimicrobial barrier function of the small bowel mucosa. Has antibacterial activity against attenuated mutants of S.typhimurium.

Involvement in disease:

Relevance:

Probably contributes to the antimicrobial barrier function of the small bowel mucosa. Has antibacterial activity against attenuated mutants of S.typhimurium.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Alpha-defensin family

Reference:

"Developmental regulation of cryptdin, a corticostatin/defensin precursor mRNA in mouse small intestinal crypt epithelium." Ouellette A.J., Greco R.M., James M., Frederick D., Naftilan J., Fallon J.T. J. Cell Biol. 108:1687-1695(1989)