Alternative Name(s):

Fibrochimerin

Species: (Organism)

Gallus gallus (Chicken)

Gene Names:

COL12A1

Tag info:

N-terminal 6xHis-tagged

Target Protein AA Sequence:

DLVFLVDGSWSVGRNNFRYILDFMVALVSAFDIGEEKTRVGVVQYSSDTRTEFNLNQYFRRSDLLDAIKRIPYKGGNTMTGEAIDYLVKNTFTESAGARKGFPKVAIVITDGKAQDEVEIPARELRNIGVEVFSLGIKAADAKELKLIASQPSLKHVFNVANFDGIVDIQNEI

Expression Region:

139-311aa

Subcellular Location:

Secreted, extracellular space, extracellular matrix

Tissue Specificity:

Type XII collagen is present in tendons, ligaments, perichondrium, and periosteum, all dense connective tissues containing type I collagen.

Protein Length:

Partial

Pathway:

Mol. Weight:

23.3 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix.

Involvement in disease:

Relevance:

Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Fibril-associated collagens with interrupted helices (FACIT) family

Reference:

Type XII collagen. A large multidomain molecule with partial homology to type IX collagen.Gordon M.K., Gerecke D.R., Dublet B., van der Rest M., Olsen B.R.J. Biol. Chem. 264:19772-19778(1989)