Alternative Name(s):

CD68

Species: (Organism)

Mus musculus (Mouse)

Gene Names:

Cd68

Tag info:

N-terminal 6xHis-SUMO-tagged

Target Protein AA Sequence:

DCPHKKAVTLLPSFTMTPTATESTASPTTSHRPTTTSHGNVTVHTSSGPTTVTHNPATTTSHGNATISHATVSPTTNGTATSPRSSTVGPHPGPPPPSPSPRSKGALGNYTWANGSQPCVQLQAQIQIRILYPIQGGRKAWGISVLNPNKTKVQGGCDGTHPHLSLSFPYGQLTFGFKQDLHQSPSTVYLDYMAVEYNVSFPQAAQWTFMAQNSSLRELQAPLGQSFCCGNASIVLSPAVHLDLLSLRLQAAQLPDKGHFGPCFSCNRDQS

Expression Region:

21-291aa

Subcellular Location:

Isoform Long: Endosome membrane, Single-pass type I membrane protein, Lysosome membrane, Single-pass type I membrane protein, SUBCELLULAR LOCATION: Isoform Short: Cell membrane, Single-pass type I membrane protein

Tissue Specificity:

Expressed in tissue macrophages and to a lesser extent in dendritic cells.

Protein Length:

Extracellular Domain

Pathway:

Mol. Weight:

44.8 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

Involvement in disease:

Relevance:

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and Extracellular domain cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma mbrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

LAMP family

Reference:

Influence of caloric restriction on motor behavior, longevity, and brain lipid composition in Sandhoff disease mice.Denny C.A., Kasperzyk J.L., Gorham K.N., Bronson R.T., Seyfried T.N.J. Neurosci. Res. 83:1028-1038(2006)