Alternative Name(s):

RecQ helicase homolog

Species: (Organism)

Mus musculus (Mouse)

Gene Names:

Blm

Tag info:

N-terminal 10xHis-tagged and C-terminal MYC-tagged

Target Protein AA Sequence:

INAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQ

Expression Region:

684-859aa

Subcellular Location:

Nucleus

Tissue Specificity:

Highly expressed in testis 12-14 days after birth (corresponding to the pachytene phase) and at much lower levels in brain, heart, liver, lung, thymus, kidney and spleen (PubMed:9840919, PubMed:27010503). Expressed in bone marrow (PubMed:27010503).

Protein Length:

Partial

Pathway:

Mol. Weight:

24.7 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Neuroscience

Function:

ATP-dependent DNA helicase that unwinds single- and double-stranded DNA in a 3'-5' direction

Involvement in disease:

Relevance:

Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE)

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Helicase family, RecQ subfamily

Reference:

"Point mutations causing Bloom's syndrome abolish ATPase and DNA helicase activities of the BLM protein." Bahr A., de Graeve F., Kedinger C., Chatton B. Oncogene 17:2565-2571(1998)