Background:

The Ras-related superfamily of guanine nucleotide binding proteins includes the R-Ras, Rap, Ral/Rec and Rho/Rab subfamilies, all of which are thought to play an important role in either endocytosis or in biosynthetic protein transport. The process of transporting newly synthesized proteins from the endoplasmic reticulum (ER) to various stacks of the Golgi complex and to secretory vesicles involves the movement of carrier vesicles and requires Rab protein function. Rab 35, also known as Rab 1C or RAY, is a 201 amino acid lipidanchored protein that is thought to function as a GTPase, playing a role in exocytotic and endocytotic pathways and functioning as an important factor in cytokinesis. Rab 35 is expressed ubiquitously and is encoded by a gene that maps to human chromosome 12, which houses over 1,100 genes and comprises approximately 4.5% of the human genome.

Specificity:

RAB35 polyclonal antibody detects endogenous levels of RAB35 protein.

Alternative names:

Ras-related protein Rab-35; GTP-binding protein RAY; Ras-related protein Rab-1C; RAB1C; RAY

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 146-191 of Human RAB35.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 23 kDa

Applications:

WB

Usage:

WB: 1:500~1:1000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com