Background:

PHKG1 (phosphorylase kinase subunit γ1), is a subunit of phosphorylase kinase (PHK) that belongs to the Ser/Thr protein kinase family. PHK is a hexadecameric protein composed of four α chains, four β chains, four γ chains and four δ chains. The γ chains are catalytic chains, the α and β chains are regulatory chains and the δ chains are calmodulins. PHKG1 contains two calmodulin-binding domains and one protein kinase domain. As the catalytic chain of PHK, PHKG1 is responsible for catalyzing the phosphorylation and activation of glycogen phosphorylase and therefore it plays an important role in the glycogenolytic pathway. Mutations in the gene encoding PHKG1 can lead to PHK deficiency and result in glycogen storage disease type 9C (GSD9C), also known as autosomal liver glycogenosis.

Specificity:

PHKG1 polyclonal antibody detects endogenous levels of PHKG1 protein.

Alternative names:

Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform; PHK-gamma-M; Phosphorylase kinase subunit gamma-1; Serine/threonine-protein kinase PHKG1; PHKG; PHK-γ-M; PHKγ-M; PHK-γM; PHKγM;

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 293-338 of Human PHKG1.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 45 kDa

Applications:

WB ICC

Usage:

WB: 1:500~1:1000 ICC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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