Background:

N4BP2L2 (NEDD4-binding protein 2-like 2), also known as PFAAP5 (phosphonoformate immuno-associated protein 5), is a 583 amino acid nuclear protein that potentially is involved in transcriptional regulation. N4BP2L2 is phosphorylated on Ser 199 in response to DNA damage, probably by ATM or ATR. Primarily expressed in bone marrow, N4BP2L2 is dramatically down-regulated after exposure to arsenic compounds, an event which precedes neutropenia. PFAAP5 interacts with both Gfi-1 and Neutrophil Elastase, two proteins that are implicated in neutropenia disorders. Defects in the gene encoding Neutrophil Elastase, ELA2, are the cause of cyclic haematopoiesis, which, with decreased numbers of circulating neutrophils, leads to an increased risk for opportunistic infection.

Specificity:

N4BP2L2 polyclonal antibody detects endogenous levels of N4BP2L2 protein.

Alternative names:

NEDD4-binding protein 2-like 2; Phosphonoformate immuno-associated protein 5; CG005; PFAAP5

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 400-445 of Human N4BP2L2.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 67 kDa

Applications:

WB

Usage:

WB: 1:500~1:1000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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