Background:

The Ets-1 family of transcription factors has a conserved DNA binding domain through which it plays an important role in cellular proliferation, differentiation, tematopoiesis and angiogenesis. This domain, also known as the Ets domain, binds to DNA sequences containing the consensus sequence 5'-WGGA-3', which is known as the Ets-binding domain. Elf-4, also known as myeloid Elf-1-like factor, ELF4 or MEF, is a 663 amino acid member of the Ets-1 family. Localized to the nucleus, Elf-4 is highly expressed in placenta and myeloid leukemia cells, with lower levels of expression lung, heart, thymus, slpeen, colon, ovary and peripheral blood lymphocytes. Functioning primarily to activate the promoters of hematopoietic growth factor genes, such as GM-CSF, IL-3 and IL-8, Elf-4 has also been shown to activate the Perforin 1 promoter in natural killer (NK) cells, suggesting a possible role in tumorigenesis.

Specificity:

Elf-4 polyclonal antibody detects endogenous levels of Elf-4 protein.

Alternative names:

ETS-related transcription factor Elf-4; E74-like factor 4; Myeloid Elf-1-like factor; ELF4; ELFR; MEF

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 219-264 of Human Elf-4.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 71 kDa

Applications:

WB

Usage:

WB: 1:500~1:1000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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