Background:

EF-1 (elongation factor-1) is a multi-protein complex that is responsible for the delivery of aminoacyl-tRNAs to the ribosome. The EF-1 protein complex is composed of four subunits: EF-1 α, EF-1 β, EF-1 δ and EF-1 γ. EF-1 β is a 225 amino acid member of the EF-1 β/EF-1 δ protein family and contains one GST C-terminal domain. Both EF-1 β and EF-1 δ stimulate the exchange of GDP bound to EF-1 α to GTP. Phosphorylation of EF-1 β affects the GDP/GTP exchange rate. Alternative splicing events of the gene that encodes EF-1 β result in three transcript variants that differ only in the 5' UTR.

Specificity:

EEF1B2 polyclonal antibody detects endogenous levels of EEF1B2 protein.

Alternative names:

Elongation factor 1-beta; EF-1-beta; EEF1B; EF1B; EF-1 β; EF-1β; EF1β; EF1-β

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 142-187 of Human EEF1B2.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 25 kDa

Applications:

WB

Usage:

WB: 1:500~1:1000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com