Background:

DNA replication, recombination and repair, all of which are necessary for genome stability, require the presence of exonucleases. In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches. Exonucleases involved in these processes include DNA polymerases, including DNA pol δ and ε. DNA pol δ consists of two subunits-p125 which interacts directly with the sliding DNA clamp protein PCNA, and p50. DNA pol δ can be regulated by cell cycle proteins. DNA pol ε is a multiple subunit enzyme, the catalytic subunit of which is encoded by the POL2 gene. The exact reactions catalyzed by DNA pol δ and ε on leading and lagging strands have not yet been elucidated.

Specificity:

DNA pol ε 2 polyclonal antibody detects endogenous levels of DNA pol ε 2 protein.

Alternative names:

DNA polymerase epsilon subunit 2; DNA polymerase II subunit 2; DNA polymerase epsilon subunit B; POLE2; DPE2; DNA pol ε2; DNA pol εB; DNA pol ε B; DNA polε2; DNApolε2; DNA polεB; DNApolεB;

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 144-189 of Human DNA pol ε 2.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 60 kDa

Applications:

WB

Usage:

WB: 1:500~1:1000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

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