Background:

The DAN (differential screening-selected gene aberrative in neuroblastoma) protein family contains antagonists of bone morphogenetic protein (BMP) signaling that are expressed in the neural crest. All family members are secreted proteins that act as BMP antagonists in embryonic explants and are expressed in the proximal airway epithelium of the lung during embryonic development. This family includes the head-inducing factor Cerberus, the dorsaling factor gremlin and the tumor suppressor DAN. DAN, also known as zinc finger protein DAN, NO3, DAND1 or neuroblastoma suppressor of tumorigenicity 1 (NBL1), is a secreted protein containing one C-terminal cysteine knot-like (CTCK) domain. It is produced in small neurons of the dorsal root ganglion and its expression is activated by MATH-1. In addition to antagonizing BMP signaling, DAN also antagonizes the action of TGFβ. DAN is a possible tumor suppressor for human neuroblastoma; defects may result in or contribute to its progression. DAN may also be a neuromodulator in inflammatory pain.

Specificity:

NBL1 polyclonal antibody detects endogenous levels of NBL1 protein.

Alternative names:

Neuroblastoma suppressor of tumorigenicity 1; DAN domain family member 1; Protein N03; Zinc finger protein DAN; DAN; DAND1

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 135-180 of Human NBL1.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 19 kDa

Applications:

WB ICC

Usage:

WB: 1:500~1:1000 ICC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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