Background:

Carbohydrate sulfotransferase 1 (CHST1), also referred to as KSGal6ST or KSST, is a keratan sulfate sulfotransferase. It is predominantly expressed in brain and skeletal muscle and localizes to the trans-Golgi network. CHST1 is responsible for mediating the sulfation of keratan in the cornea, which is important in maintaining corneal transparency. In particular, CHST1 catalyzes the transfer of sulfate groups from 3’-phosphoadenosine 5’-phosphosulfate to position six of internal or terminal galactose residues (preferentially the residues adjacent to sulfated GlcNAc) on growing keratan sulfate chains. CHST1 also contributes to the generation of L-selectin ligands. Mutations in the CHST1 gene may play a role in macular corneal dystrophy (MCD).

Specificity:

CHST1 polyclonal antibody detects endogenous levels of CHST1 protein.

Alternative names:

Carbohydrate sulfotransferase 1; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1; GST-1; Keratan sulfate Gal-6 sulfotransferase; KS6ST; KSGal6ST; KSST

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 126-171 of Human CHST1.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 47 kDa

Applications:

WB

Usage:

WB: 1:500~1:1000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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