Background:

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (α, βI, βII and γ) and novel (n) PKC isoforms (δ, ε, ζ, η and θ). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC δ and ε are independent of Ca2+.

Specificity:

p-PKC-pan (αT497) polyclonal antibody detects endogenous levels of PKC PKC α, β I, β II, γ, δ, ε, η, ζ and θ isoforms only when phosphorylated at a residue homologous to threonine 497 of human PKCα.

Alternative names:

Protein kinase C alpha type; PKC-A; PKC-alpha; PRKCA; PKCA; PRKACA; Protein kinase C beta type; PKC-B; PKC-beta; PKCB; PRKCB1; PRKCB; Protein kinase C gamma type; PKC-gamma; PKCG; PRKCG; Protein kinase C delta type; Tyrosine-protein kinase PRKCD; nPKC-delta; Sphingosine-dependent protein kinase-1; SDK1; PRKCD; Protein kinase C epsilon type; nPKC-epsilon; PRKCE; PKCE; Protein kinase C zeta type; nPKC-zeta; PRKCZ; PKC2; PKC zeta; Protein kinase C theta type; nPKC-theta; PRKCQ; PRKCT

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic phosphopeptide derived from human PKC-pan around the phosphorylation site of Threonine 497.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~75 to 88 kDa

Applications:

WB IHC

Usage:

WB: 1:500~1:1000 IHC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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