Background:

The p95 gene (identical to NBS1 and nibrin) is a member of the hMre11/hRad50 double-strand break complex (MRN complex). This protein complex has been implicated in Nijmegen breakage syndrome, an autosomal recessive disorder marked by increased cancer incidence, cell cycle checkpoint deficits, and ionizing radiation sensitivity, thus revealing a direct molecular link between double-strand break repair and cell cycle checkpoint functions. In case of infection by adenovirus E4, the MRN complex is inactivated and degraded by viral oncoproteins, thereby preventing concatenation of viral genomes in infected cells. NBS1 is expressed ubiquitously and presents high levels in testis.

Specificity:

p-p95/NBS1 (S343) polyclonal antibody detects endogenous levels of p95/NBS1 protein when phosphorylated at Ser343.

Alternative names:

Nijmegen breakage syndrome protein 1; Cell cycle regulatory protein p95; NBS1/P95; NBS; NBS1; P95

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic phosphopeptide derived from human Nibrin around the phosphorylation site of Serine 343.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 95 kDa

Applications:

WB IHC

Usage:

WB: 1:500~1:1000 IHC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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