Background:

The core of PDC is composed of sixty dihydrolypoyl acetyltransferase (E2) subunits that bind directly to PDK2 and enhance PDK2 kinase activity. Upregulation of PDK isoenzymes occurs during starvation conditions, rerouting acetyl-CoA generation by facilitating fatty acid oxidation. PDKs contain five conserved regions and are mechanistically similar to bacterial His-kinases, in that both require histidine residues for activity. In mammals, transcripts for PDK2 are ubiquitously expressed with high levels in heart and skeletal muscle and decreased levels in spleen and lung.

Specificity:

PDK2 (R291) polyclonal antibody detects endogenous levels of PDK2 protein.

Alternative names:

[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2; mitochondrial; PDHK2; PDK2; PDK2; Pyruvate dehydrogenase kinase isoform 2; Pyruvate dehydrogenase kinase; isozyme 2; Pyruvate dehydrogenase lipoamide kinase isozyme 2; mitochondrial

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 260-322 of Human PDK2.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 46 kDa

Applications:

WB IHC IP

Usage:

WB 1:500 - 1:1000 IHC 1:50 - 1:100 IP 1:10 - 1:100

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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