Background:

Mammalian mitochondrial ribosomes (mitoribosomes) are responsible for protein synthesis within the mitochondrion. The mitoribosomes are composed of a 4:1 ratio of protein to RNA, with the proteins forming two subunits, the 28S subunit and the 39S subunit. Across species, the proteins that make up the mitoribosome subunits vary greatly in sequence, preventing easy recognition by sequence homology. MRP-S9 (mitochondrial 28S ribosomal protein S9), also known as RPMS9, is a 396 amino acid mitochondrial ribosomal protein. Localized to mitochondria, MRP-S9 is present in the 28S subunit of the mitoribosomes, which comprises a 12S rRNA and at least 30 distinct proteins.

Specificity:

MRP-S9 (F132) polyclonal antibody detects endogenous levels of MRP-S9 protein.

Alternative names:

28S ribosomal protein S9, mitochondrial; MRP-S9; S9mt; MRPS9; RPMS9

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 100-150 of Human MRP-S9.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 46 kDa

Applications:

WB IHC

Usage:

WB: 1:500~1:1000 IHC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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