Background:

Interleukin 5 (IL-5) is a soluble T cell-derived factor, also known as T cellreplacing factor (TRF), that induces B cell and eosinophil growth and differentiation. IL-5 exerts its biological activity through the IL-5 receptor (IL-5R),which is composed of two chains: an α chain that binds IL-5 with low affinity and a β chain that does not bind IL-5, but together with the IL-5 α chain,constitutes the high affinity IL-5 receptor. The cytoplasmic domain of both the α and β chains is essential for signal transduction. Specifically, the membrane-proximal proline-rich sequence of the ytoplasmic domain of the IL-5R receptor α subunit, IL-5Rα, is critical for the IL-5 induced proliferative response, expression of nuclear proto-oncogenes and tyrosine phosphorylation of cellular proteins, such as JAK1 and JAK2.

Specificity:

IL-5Rα (K68) polyclonal antibody detects endogenous levels of IL-5Rα protein.

Alternative names:

Interleukin-5 receptor subunit alpha; IL-5 receptor subunit alpha; IL-5R subunit alpha; IL-5R-alpha; IL-5RA; CDw125; CD125; IL5RA; IL5R

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 50-100 of Human IL-5Rα.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 48 kDa

Applications:

IHC

Usage:

IHC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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