Background:

Pax-1 is a sequence-specific DNA binding-protein with transcriptional activating properties. The expression pattern of Pax-1 during mouse embryogenesis indicates that it may play an important role in the development of the vertebral column. The autosomal recessive mutation ‘undulated’ (un) in the mouse exhibits vertebral anomalies along the entire rostrocaudal axis and is associated with a point mutation (G-to-A transition) at position 15 leading to a gly-to-ser replacement in a highly conserved region of the paired box of Pax-1. Pax-1 is required for the normal development of these three skeletal elements: the vertebral column, sternum, and scapula. Mice who are doubly heterozygous for the mutants ‘undulated’ and ‘Patch’ have a phenotype reminiscent of an extreme form of spina bifida occulta in humans.

Specificity:

Pax-1 (T367) polyclonal antibody detects endogenous levels of Pax-1 protein.

Alternative names:

HuP48; Paired box protein Pax-1; PAX1; HUP48

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 330-380 of Human Pax-1.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 55 kDa

Applications:

IHC

Usage:

IHC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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