Background:

Mammalian protein farnesyl transferases are heterodimeric proteins containing two nonidentical α and β subunits that attach farnesyl residues to a cysteine at the fourth position from the COOH terminus of several proteins, including nuclear lamins and p21Ras proteins. The natural substrates contain the Cys-A-A-Xaa recognition sequence, where the A residues are aliphatic and Xaa represents methionine, serine, glutamine or cysteine. The purified farnesyl transferase is an a-b heterodimer. The β subunit, which is known as FTβ, CAAX farnesyltransferase subunit β, or Ras proteins prenyltransferase subunit β, is a 437 amino acid protein that contains five PFTB repeats and binds the peptide substrate. The α subunit is suspected to participate in formation of a stable complex with the substrate farnesyl pyrophosphate.

Specificity:

FTβ (E285) polyclonal antibody detects endogenous levels of FTβ protein.

Alternative names:

Protein farnesyltransferase subunit beta; FTase-beta; FTase-β; FTase β; CAAX farnesyltransferase subunit beta; Ras proteins prenyltransferase subunit beta; FNTB

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 250-300 of Human FTβ.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 48 kDa

Applications:

WB

Usage:

WB: 1:500~1:1000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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