Background:

Reacts with HLA-DO, a heterodimer formed by DNα and DOβ subunits in B cells. DNα and DOβ are the products of the non-classical class II genes, HLA-DNA and HLA-DOB, respectively. DO forms tight complexes with DM in the endoplasmic reticulum and is thereby sorted to lysosomal vesicles during antigen processing and presentation. It is tightly associated with DM and it is selectively expressed on antigen-presenting cells, such as B cells, dendritic cells and thymic epithelial cells. DO can enhance the efficiency of peptide loading and has been found to stabilize DM at low pH, preserving its chaperon activity. DO-DM complexes are more efficient than DM in protecting empty DR molecules. Reports describe DO as a co-chaperone of DM. This antibody has also been reported to be suitable for western blot and immunoprecipitation.

Specificity:

HLA-DOα (V111) polyclonal antibody detects endogenous levels of HLA-DOα protein.

Alternative names:

HLA class II histocompatibility antigen; DO alpha chain; MHC DN-alpha; MHC DZ alpha; MHC class II antigen DOA; HLA-DNA; HLA-DZA; HLA-DOA

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 81-130 of Human HLA-DOα.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 34 kDa

Applications:

IHC

Usage:

IHC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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